English Türkçe

Quick Access


Bu Dergi DOI ve Crosscheck üyesidir


Samsun, Sinop ve Bolu illerinden toplanan bal kabağı (Cucurbita moschata Duch) populasyonlarına ait tohum örneklerinde virüslerin tanılanması ve yaygınlıklarının belirlenmesi
(Detection and incidence of viruses in the seeds of pumpkin (Cucurbita moschata Duch) populations collected from Samsun, Sinop and Bolu provinces )

Author : Mehmet Ali ŞEVİK  Ahmet BALKAYA  
Type :
Printing Year : 2015
Number : 2015-3
Page : 70-77


Summary
Bal kabağı, Cucurbitaceae familyasında yer alan önemli sebze türlerinden biridir. Bu familya içinde yer alan türler viral hastalıklara karşı oldukça duyarlıdır ve 39’dan fazla virüs bu bitkilerin ekonomik olarak verimliliklerini etkileyebilmektedir. Bu çalışma, bal kabağı populasyonlarında tohum kaynaklı virüslerin belirlenmesi amacıyla 2013-2014 yıllarında yürütülmüştür. Samsun, Sinop ve Bolu illerinden toplanan bal kabağı populasyonlarına ait örneklerde tohum kaynaklı virüsleri belirlemek amacıyla Double Sandwich-Enzyme linked Immunosorbent Assay (DAS-ELISA) yöntemi kullanılmıştır. Tohum örnekleri Cucumber mosaic virus (CMV), Zucchini yellow mosaic virus (ZYMV), Squash mosaic virus (SqMV), Tobacco ring spot virus (TRSV) ve Cucumber green mottle mosaic virus (CGMMV) için ELISA yöntemi ile analiz edilmiştir. ELISA sonuçlarına göre, tohum örneklerinin sadece ZYMV ve CMV ile enfekteli olduğu belirlenmiştir. Analiz sonucunda, bal kabağı tohum örneklerinde ZYMV yoğunluğu %12.5 olarak belirlenirken, CMV’nin bulunma oranı %4.1 olarak saptanmıştır. Ancak, SqMV, TRSV ve CGMMV ile bulaşık hiçbir balkabağı tohum örneğine rastlanmamıştır. Bu çalışma sonucunda, Karadeniz Bölgesi’nde bal kabağı populasyonlarına ait tohum örneklerinde ilk kez tohum kaynaklı virüslerin tanılanması yapılmış ve yaygınlık durumları belirlenmiştir.

Turkish Keywords
Bal kabağı, Karadeniz Bölgesi, tanılama, tohum, virüs

Abstract
Pumpkin is one of the most important vegetables in Cucurbitaceae family. Species belonging to this family are susceptible to a number of viral diseases and economically affected by more than 39 viruses. This study was carried out to detect the presence of seed borne viruses in pumpkin populations during 2013-2014. Double sandwich-enzyme linked immunosorbent assay (DAS-ELISA) was used to detect seed borne viruses in the populations collected from Samsun, Sinop, and Bolu provinces. The seed samples were tested by ELISA for Cucumber mosaic virus (CMV), Zucchini yellow mosaic virus (ZYMV), Squash mosaic virus (SqMV), Tobacco ring spot virus (TRSV), and Cucumber green mottle mosaic virus (CGMMV). Based on the ELISA results, the seed samples were only infected with ZYMV and CMV. According to the results of ELISA, CMV was found at rate of 4.1% in pumpkin seed samples, while incidence of ZYMV was determined as 12.5%. Moreover, none of the seed samples were found to be infected for SqMV, TRSV, and CGMMV. At the end of this study, this is the first time that seed-borne viruses of pumpkin populations in the Black Sea Region of Turkey have been identified and determined the prevalence of these viruses.

Keywords
Pumpkin, The Black Sea Region, identification, seed, virus

Advanced Search


Announcements















    [JAFAG] Yeni Sayımız Basılmıştır JAFAG, 34 (2)

    [JAFAG] New Issue Published JAFAG, 34 (2)



    Call for papers

    To Researcher:

    I am delighted to invite you to disseminate your research findings in Journal of Agricultural Faculty of Gaziosmanpasa University (JAFAG).  I wish to publish original research articles in agricultural sciences. In our journal: rapid manuscript publication and peer review process with online manuscript submission system, quick access of your research results to global audience  with open access system.

    I hope you will contribute your article for publication in JAFAG and thanking you in advance.


    AIMS and SCOPE

    Journal of Agricultural Faculty is scientific, peer reviewed journal and belonged to the Gaziosmanpasa University Faculty of Agriculture since 1985. Journal of Agricultural Faculty of Gaziosmanpasa University aims to publish the national and international scientific paper on agriculture sciences (agricultural economics, animal science, biosystems engineering, field crops, fisheries engineering, horticulture, plant protection, soil science and plant nutrition). The journal is published the third issues in a year from 2014.


    Journal of Agricultural Faculty of Gaziosmanpasa University (JAFAG)

    Journal of Agricultural Faculty of Gaziosmanpasa University (JAFAG) is an international peer-reviewed journal and belonged to the Gaziosmanpasa University Faculty of Agriculture since 1985.

    The journal is open access.

    The journal has both online and printed publishing.

    The journal has a manuscript submisson system.

    There are no page charges.


    INDEXING

    ACADEMIC JOURNALS DATABASE

    AGRICOLA

    ARASTIRMAX

    CAB ABSTRACTS

    CROSSREF

    DIRECTORY OF SCIENCE

    DOAJ (DIRECTORY OF OPEN ACCESS JOURNALS)

    DRJI (DIRECTORY OF RESEARCH JOURNAL INDEXING)

    EBSCO

    EFITA (INTERNATIONAL DIRECTORY OF AGRICULTURE, FOOD AND THE ENVIRONMENT)

    ELECTRONIC JOURNALS LIBRARY

    FAO- AGRIS

    GENAMICS JOURNALSEEK

    GLOBAL IMPACT AND QUALITY FACTOR

    GOOGLE SCHOLAR

    INDEX COPERNICUS

    JOURNAL INDEX

    JOUR INFORMATICS

    OPEN ACCESS LIBRARY

    OPEN J-GATE

    RESEARCH BIBLE

    ROAD (DIRECTORY OF OPEN ACCESS SCHOLARLY  RESOURCES)

    SIS (SCIENTIFIC INDEXING SERVICE)

    SCIENCE CENTRAL

    THE GROVE ONLINE LIBRARY

    TUBITAK-ULAKBIM

    ULRICHSWEB

    WORLDCAT


    [JAFAG] New Issue Published JAFAG, 34 (2)

    Journal of Agricultural Faculty of Gaziosmanpasa University, JAFAG has just published its latest issue (Vol 34, No 2, 2017, JAFAG) at http://ziraatdergi.gop.edu.tr.

    ICV Impact Factor: 4.95 (2012) - 5.94 (2013) - 70.06 (2016)

    We invite you to review the Contents here and then visit journal web site to review articles.

    Thanks for the continuing interest in our work in journal.



Address :Gaziosmanpaşa Üniversitesi Ziraat Fakültesi Dergisi Yayın Ünitesi, Tokat-TÜRKİYE / Adress:Journal of Agricultural Faculty of Gaziosmanpasa University Publishing Unit, Tokat, TURKEY
Telephone :03562521616-2178 / Telephone: +90 356 252 16 16- 2 Fax :03562521488 / Fax:+90 356 252 14 88
Email :ziraatdergi@gop.edu.tr/ e-mail: ziraatdergi@gop.edu.tr

Web Yazılım & Programlama Han Yazılım Bilişim Hizmetleri