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Aşılı asma çeliklerinin histolojik incelenmesinde farklı fiksasyon ve boyama yöntemlerinin etkileri
(The effects of fixation and staining methods in histological investigation on the grafted cuttings of grapevine )

Author : Cuma ARIK  Ahmet ALTINDİŞLİ  
Type :
Printing Year : 2021
Number : 2021-1
Page : 65-72


Summary
Aşılı asma üretiminde aşı kaynaşması ve diğer aşı özelliklerinin incelenmesi amacıyla, histolojik çalışmalar yürütülmektedir. Histolojik inceleme için, aşı örnekleri bir fiksasyon çözeltisinde muhafaza edilmekte ve sonrasında bu aşı örneklerinden alınan aşı kesitleri bazı histolojik boyama yöntemleri ile boyanmaktadır. Asma aşı örneklerinin histolojik incelemesinde daha once uygulanmamış bir fiksasyon yönteminin ve farklı boyama yöntemlerinin çalışılmış olması, bu çalışmanın önemini ortaya koymaktadır. Asma aşı örneklerinin fiksasyonunda genel olarak %70 etil alkol ve FAA (formaldehit: glasiyel asetik asit: alkol) fiksatifleri kullanılmaktadır. Fakat bir diğer fiksatif çözelti, gliserin: alkol:su lignoselülozik doku fiksasyonunda kullanılmaktadır. Bu çalışmada bu fiksasyon yöntemlerinin asma aşı örneklerinin fiksasyonundaki farklı etkilerinin saptanması amaçlanmıştır. Sonuç olarak gliserin:alkol:su fiksasyon metodunun asma aşı örneklerinin fiksasyonu için önerilebilir bulunmuştur. Ayrıca toluidin mavisi, safranin + hematoksilen ikili boyama ve safranin + Lugol ikili boyama gibi farklı histolojik boyama yöntemleri karşılaştırılmıştır; toluidin mavisi en başarılısı olmuş ve bunu safranin + Lugol ikili boyama yöntemi izlemiştir. Aşı özelliklerinin (anaç ve kalem arasındaki kambiyal bağlantı ve vasküler farklılaşma gibi) daha net boyanmasından dolayı, aşı kesitlerinin boyanmasında toluidin mavisi önerilebilir bulunmuştur.

Turkish Keywords
Toluidin mavisi, safranin, lugol, hematoksilen, gliserin

Abstract
For purpose of investigation of graft union and other graft properites in grafted grapevine production, the histological studies are conducted. For this, graft samples are stored in a fixation solution and later sections are stained by using some histological staining methods. Being examined of previously unapplied a fixation method and different staining methods has constituted the importance of this study, in histological investigation of grapevine graft samples. In grapevine graft samples fixation, 70% ethyl alcohol and formaldehyde: glacial acetic acid: alcohol fixatives have been used in general. But also, another fixative solution, glycerin: alcohol: water solution is used for lignocellulosic tissue fixation. In this study, observing dissimilar effects between these fixation methods in grapevine graft samples fixation was aimed. As a result, it’s thought that, glycerin: alcohol: water fixation method may be suggestible for grapevine graft samples fixatiation. Furthermore, different histological staining methods as toluidine blue, safranin + hematoxylin dual staining and safranin + Lugol’s solution dual staining were compared; toluidine blue was forefront and safranin + Lugol’s solution was second. And it's seen that, because of staining graft properties (continuity of cambium and vascular differentiation etc.) clearer, toluidine blue is suggestible for graft section staining.

Keywords
Toluidine blue, safranin, lugol, hematoxylin, glycerin

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