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Besin ortamı, stres koşulları ve genotipin biberde androgenik başarı üzerine etkisi
(Effect Of Media, Stress Conditions And Genotype On Androgenıc Performance Of Pepper )

Author : Sevtap DOKSÖZ BONCUKÇU  Murat Emre Çelik, Naif Geboloğlu  
Type :
Printing Year : 2020
Number : 2020-1
Page : 23-29


Summary
Çalışmada üç burun meyve yapısına sahip biber genotiplerinde genotip, besi ortamı, stres ve inkübasyon uygulamalarının anter kültürü yöntemiyle haploid bitki oluşumuna etkileri araştırılmıştır. Denemede donör bitki olarak İstek F1, Köylüm F1, Üçburun F2 ve yerel genotip olan Tokat biberi kullanılmıştır. DDV(Dumas de Vaulx ve ark. ,1981) ve MS(Murashige ve Skoog, 1962) olarak bilinen ortamlar besi ortamı olarak kullanılmıştır. Ortamlara 0,01 mg/l Kinetin + 0.01 mg/l 2.4D + 0.03 mg/l Vitamin B12 ilave edilmiştir. Çiçek tomurcukları 4 oC’de 24 saat bekletilmiştir. Kontrol bitkilerinin tomurcuklarına düşük sıcaklık uygulaması yapılmamıştır. Anterler, besi ortamına ekildikten sonra 9 oC ve 35 oC’de 8 gün süreyle karanlık ortamda bekletilmiş ardından 16 saat gündüz/8 saat gece ve 25±2 oC sıcaklığa sahip ortama alınmıştır. Bu ortamda 4 gün bekleyen anterler daha sonra rejenerasyon ortamına aktarılmıştır. Denemede kullanılan genotiplerin hepsi az ya da çok anter kültürüne cevap vermişlerdir. En yüksek embriyo oluşumu yerel Tokat genotipinden elde edilmiştir. Embriyo oluşum oranları uygulamalara göre % 0.25 ile % 31.00 arasında değişmiştir. MS besi ortamına ekilen anterlerden embriyo oluşum oranı % 3.9 olurken, DDV ortamına ekilen anterlerden embriyo elde etme oranı % 9.33 olmuştur. Ön uygulamaya tabi tutulan anterlerde embriyo oluşum oranı % 6.7 olurken, kontrol uygulamasında % 6.4 olmuştur. Çalışmada 9 oC inkübasyon uygulamasında embriyo oluşum oranı % 7.60 olurken, 35 oC’de inkübasyon uygulamasında embriyo oluşum oranı % 5.50 olmuştur.

Turkish Keywords
Anter kültürü,DDV, genotip, inkübasyon, MS, stres,

Abstract
This study was conducted with the purpose of investigation of the effects of genotype, nutrient media, stress and incubation treatments on haploid plant development on some three-lobed pepper genotypes. İstek F1, Köylüm F1, Üçburun F2 and Tokat local genotype were used as donor plant. DDV (Dumas de Vaulx et al. ,1981) and MS (Murashige and Skoog, 1962) were used as nutrient media. Furthermore, 0.01 mg L-1 Kinetin + 0.01 mg L-1 2,4D + 0.03 mg L-1 Vitamin B12 were added to the media. Flower buds were held at 4 oC for 24 hours. Low-temperature treatment was not applied on flower buds of control plants. After anthers were placed in the nutrient media, they were held at 9 oC and 35 oC for 8 hours in the darkness. Afterwards, anthers were held at 25±2 oC for 16 h day / 8 h night for 4 days and transferred to regeneration media. All genotypes that were used in the experiment gave a response to the anther culture more or less. The highest embryo development was obtained from Tokat genotype. Embryo development rates varied between 0.25% and 31.00% by treatments. While embryo development rate was 3.9% in MS medium, it was 9.33% in DDV medium. Moreover, while embryo development rate was 6.7% on anthers that were exposed to cold pre-treatment, it was 6.4% in the control treatment. Embryo development rate in 9 oC and 35 oC incubation treatments were 7.60% and 5.50% respectively.

Keywords
Anther culture, DDV, genotype, incubation, MS, stress

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