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Old Home x Farmingdale 333 Armut Anacının in Vitro Köklenmesi Üzerine Oksin ve Polivinil Alkol Uygulamalarının Etkileri
(Effects of Auxin and Polyvinyl Alcohol Treatments on in Vitro Rooting of Old Home x Farmingdale 333 Pear Rootstock )

Author : Hatice DUMANOĞLU  Shabnam ALİZADEH Gülüstan POLAT  
Type :
Printing Year : 2018
Number : 2018-Ek Sayı
Page : 47-53


Summary
Bu çalışmada, Old Home x Farmingdale 333 (OHxF 333) (Pyruscommunis L.) armut anacında mikro çeliklerin in vitro köklenmesi üzerine PVA’nın (polivinil alkol), IBA (indolbütirik asit) ve IAA (indolasetikasit)’nın etkileri araştırılmıştır. PVA denemesinde (1. deneme), aseptik koşullarda, hızlı daldırma yöntemiyle 200 ppm IBA uygulanmış mikro çelikler, 0, 1, 2 ve 3 gl-1 PVA ilave edilmiş, büyümeyi düzenleyici madde içermeyen, makro element düzeyi ½ olan MS (Murashige ve Skoog) temel besin ortamı üzerinde kültüre alınmıştır. Oksin denemesinde (2. deneme) ise IBA ve IAA’nın 0, 12, 24, 48, 120 ve 240 ppm dozlarının aseptik koşullarda yavaş daldırma yöntemiyle (1 saat) uygulandığı mikro çelikler, PVA içermeyen aynı temel besin ortamına dikilmiştir. Kültürler bir hafta karanlık, beş hafta 16 saat aydınlık koşullarda tutulmuştur. Çalışmanın sonucunda, hızlı daldırma yöntemiyle 200 ppm IBA uygulanmış mikro çeliklerde PVA (1, 2 ve 3 g l-1)’nın invitro köklenme oranlarını önemli düzeyde artırdığı (%75-100) belirlenmiştir. Köklenme düzeyi (ortalama 2.51), ortalama kök sayısı (ortalama 3.54 adet) ve kök uzunluğu (ortalama 17.0 mm) bakımından uygulamalar arasında istatistiksel anlamda önemli bir farklılık ortaya çıkmamıştır. Oksin denemesinde ise en yüksek köklenme oranları IAA’nın 48, 120, 240 ppm ve IBA’nın 12, 24, 120 ppm uygulamalarında %50.0 ile %83.3 arasında kaydedilmiştir.

Turkish Keywords
Pyrus, mikro çoğaltım, in vitro, köklenme, PVA

Abstract
In this study, effects of polyvinyl alcohol (PVA), IBA (indole-3-butyric acid) and IAA (indole-3-acetic acid) on in vitro rooting of micro cuttings in Old Home x Farmingdale 333 (OHxF 333) (Pyruscommunis L.) pear rootstock. In the PVA experiment (Trial 1), micro cuttings, dipped in 200 ppm of IBA by quick-dip method under aseptic conditions, were cultured on MS (Murashige and Skoog) basal medium containing half strength of macronutrients without plant growth regulators and added PVA of 0, 1, 2 and 3 g l-1. In the auxin experiment (Trial 2), micro cuttings, dipped in 0, 12, 24, 48, 120 and 240 ppm concentrations of IBA or IAA by dilute solution soaking method during an hour under aseptic conditions, were cultured on same basal medium without PVA.The cultures were incubated for a week in the dark and then for five weeks under 16-h photoperiod conditions. As a result of the study, it was determined that PVA (1, 2 and 3 g l-1) significantly increased in vitro rooting rates (75-100%) of micro cuttings with 200 ppm IBA applied by quick-dipping method. Differences between the levels of rooting (mean 2.51), the average number of roots (mean 3.54) and root length (mean 17.0 mm) were not statistically significant. In the auxin experiment, the highest rooting rates were recorded between 50.0% and 83.3% in the applications of 48, 120, 240 ppm of IAA and 12, 24, 120 ppm of IBA.

Keywords
Pyrus, micropropagation, in vitro, rooting, PVA

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