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Tıbbi öneme sahip Peganum harmala L. bitkisinin farklı eksplantlarında çoklu sürgün ve kallus oluşumu
(Multiple shoot and callus formation in different explants of the medicinally important Peganum harmala L. )

Author : Burcu ÇETİN  Halil İbrahim KOÇAK  
Type :
Printing Year : 2020
Number : 2020-2
Page : 77-83


Summary
Peganum harmala L. içerdiği metabolitler nedeniyle tıbbi öneme sahiptir. Bitki doku kültürü yöntemleri ile tıbbi bitkilerin üretimi konusunda çalışmaların yapılması önem verilmelidir. Bu çalışmada, P. harmala bitkilerinden izole edilen hipokotil ve sürgün ucu eksplantlarına farklı konsantrasyonlarda ve kombinasyonlarda benzil amino pürin (BAP), naftalen asetik asit (NAA) ve indol asetik asit (IAA) bitki büyüme düzenleyicileri uygulanmıştır. En iyi kallus gelişimi hipokotil eksplantları 1 mg L-1 NAA ve 1 mg L-1 BAP içeren Murashige ve Skoog (MS) besin ortamında elde edilmiştir. Eksplant başına düşen sürgün sayısı (5.42±0.32 adet) ve sürgün uzunluğu (2.95±0.06 cm) bakımından en iyi sonuçlar 1.5 mg L-1 BAP içeren MS besin ortamında belirlenmiştir. Elde edilen sürgünlerin köklendirilmesi için IBA uygulanmıştır. Köklenen bitkiler %72 başarı ile toprağa aktarılmışladır.

Turkish Keywords
Peganum harmala L., bitki büyüme düzenleyici, bitki doku kültürü, mikroçoğaltım

Abstract
Peganum harmala L. is medicinally important due to the metabolites it includes. It is significant that studies on the production of medicinal plants with plant tissue culture methods are being carried out. In this study, hypocotyl and shoot tips isolated from P. harmala plants are applied different concentrations and combinations of benzyl amino purine (BAP), naphthalene acetic acid (NAA), and indol acetic acid (IAA) plant growth regulators. Best callus growth hypocotyl explants were obtained in Murashige and Skoog (MS) medium that contains 1 mg L-1 NAA and 1 mg L-1 BAP. Best results concerning shoot count per explant (5.42±0.313 count) and shoot length (2.95±0.06 cm) were determined in MS medium which contains 1.5 mg L-1 BAP. IBA applications were performed in order to root the obtained shoots. The rooted plants were transferred to soil with 72% survival rate.

Keywords
Peganum harmala L., plant growth regulator, plant tissue culture, micropropagation

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