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Mikroalg üretimi için Haematococcus pluvialis türünün flashing yöntemi ile incelenmesi
(Investigation of Haematococcus pluvialis for microalgae cultivation using the flashing light method )

Author : Ahmet Konuralp ELİÇİN    
Type :
Printing Year : 2017
Number : 2017-1
Page : 264-270


Summary
Bu çalışmada, biyoyakıt üretimine uygunluk, yağ oranı miktarının fazla olması, kolay bulunabilirlik ve kontaminasyona dayanıklılık gibi faktörler göz önüne alınarak, Chlorophyta sınıfına ait Haematococcus pluvialis  mikroalg türü seçilmiştir. İncelenmeye alınan bu türün; flashing (yanıp sönen) yöntemi ile zamana dayalı voltaj, voltaja bağlı ışık yoğunluğu kalibrasyon çalışması ve zamana bağlı hücre yoğunluğundaki artış miktarları belirlenmiştir. Deneme yönteminde yapılan voltaj kalibrasyon çalışmaları sonucunda flashing uygulaması ışık şiddeti 850 µE m-2s-1 olarak belirlenmiştir. Sürekli ve eş yoğunlukta ışık şiddeti uygulayabilmek için FMT-150 tipi fotobiyoreaktör kullanılmış ve mikroalg türünün fotobiyoreaktöre verildiği ilk gün 350 mg ml-1 hücre sayımı yapılmıştır. Başlangıç süreci ile birlikte her 48 saatte bir 25oC sabit sıcaklıkta hücre sayımları yapılarak büyüme süreci incelenmiştir. Tüm parametreler uygulandıktan sonra 350 mg ml-1 hücre yoğunluğuna sahip Haematococcus pluvialis türü 32. günün sonuna kadar büyüme eğilimi göstermiştir. En yüksek hücre sayısına 32. günde 565 mg ml-1 ile ulaşılırken, 32. günden sonra popülasyonda düşme görülmeye başlanmış ,38. günde deneme sonlandırılmıştır.

Turkish Keywords
Biyodizel, Haematococcus pluvialis, mikroalgler, yenilenebilir enerji kaynakları

Abstract
In view of factors such as their suitability for biofuel production, high oil content, easy accessibility and resistance to contamination, Haematococcus pluvialis species of Chlorophyta class was the choice of microalgae selected for this study. Time-based voltage, voltage-based light intensity calibration study and the amount of increase in cell concentration in time have been specified using the flashing method for the examined species. As a result of voltage calibration studies conducted under the trial method, light intensity for flashing practice was detected as 850 µE m-2s-1. In order to apply light intensity continuously and at an equivalent level, a FMT-150 photobioreactor was employed and on the first day when the microalgae species was introduced into the photobioreactor, a cell count of 350 mg/ml-1 was obtained. The growth process was examined through the cell counts carried out every 48 hours, including the initial phase, under a constant temperature of 25oC. Having applied all these parameters, the Haematococcus pluvialis species, which had a cell concentration of 350 mg ml-1, displayed a tendency to grow until the end of the 32th day. While the highest number of cells was reached on the 32nd day with 565 mg mL-1, the population started to decline after the 32nd day, and the trial was terminated on the 38th day.

Keywords
Biodiesel, Haematococcus Pluvialis, microalgae, renewable energy sources

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