Abstract
Effects of Auxin and Polyvinyl Alcohol Treatments on in Vitro Rooting of Old Home x Farmingdale 333 Pear Rootstock
In this study, effects of polyvinyl alcohol (PVA), IBA (indole-3-butyric acid) and IAA (indole-3-acetic acid) on in vitro rooting of micro cuttings in Old Home x Farmingdale 333 (OHxF 333) (Pyruscommunis L.) pear rootstock. In the PVA experiment (Trial 1), micro cuttings, dipped in 200 ppm of IBA by quick-dip method under aseptic conditions, were cultured on MS (Murashige and Skoog) basal medium containing half strength of macronutrients without plant growth regulators and added PVA of 0, 1, 2 and 3 g l-1. In the auxin experiment (Trial 2), micro cuttings, dipped in 0, 12, 24, 48, 120 and 240 ppm concentrations of IBA or IAA by dilute solution soaking method during an hour under aseptic conditions, were cultured on same basal medium without PVA.The cultures were incubated for a week in the dark and then for five weeks under 16-h photoperiod conditions.
As a result of the study, it was determined that PVA (1, 2 and 3 g l-1) significantly increased in vitro rooting rates (75-100%) of micro cuttings with 200 ppm IBA applied by quick-dipping method. Differences between the levels of rooting (mean 2.51), the average number of roots (mean 3.54) and root length (mean 17.0 mm) were not statistically significant. In the auxin experiment, the highest rooting rates were recorded between 50.0% and 83.3% in the applications of 48, 120, 240 ppm of IAA and 12, 24, 120 ppm of IBA.
Keywords
Pyrus, micropropagation, in vitro, rooting, PVA
