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Abstract


Multiple shoot and callus formation in different explants of the medicinally important Peganum harmala L.
Peganum harmala L. is medicinally important due to the metabolites it includes. It is significant that studies on the production of medicinal plants with plant tissue culture methods are being carried out. In this study, hypocotyl and shoot tips isolated from P. harmala plants are applied different concentrations and combinations of benzyl amino purine (BAP), naphthalene acetic acid (NAA), and indol acetic acid (IAA) plant growth regulators. Best callus growth hypocotyl explants were obtained in Murashige and Skoog (MS) medium that contains 1 mg L-1 NAA and 1 mg L-1 BAP. Best results concerning shoot count per explant (5.42±0.313 count) and shoot length (2.95±0.06 cm) were determined in MS medium which contains 1.5 mg L-1 BAP. IBA applications were performed in order to root the obtained shoots. The rooted plants were transferred to soil with 72% survival rate.

Keywords
Peganum harmala L., plant growth regulator, plant tissue culture, micropropagation


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