English Türkçe

Quick Access


Bu Dergi DOI ve Crosscheck üyesidir


Abstract


Determination of Kinetic Parameters of Microbial Dipeptidyl Peptidase IV
Dipeptidyl peptidase IV (EC 3.4.14.5; DPPIV) is an important enzyme that has role for regulation of tissue and organ systems and been investigated for neurological, immunological and gastroenterological aspects. DPPIV is expressed as regulator enzyme by mammalian, plant, insect and microbial cells. Recently, commercial DPPIV enzyme production has increased via using as dietary supplement for people who suffer from deficiency of digestion enzyme, autism, celiac and Asperger syndrome. Although fungal resources which are expressing extracellular enzyme were preferred to produce DPPIV, production of DPPIV is recently performed by bacteria; because, productivity values are generally higher in bacteria than fungus. In this study, Lactococcus lactis spp. lactis (L. lactis) was used as an enzyme producer microorganism. L. lactis expresses endo– and exo-peptidases which DPPIV is one of them to hydrolyze casein. Intracelluler DPPIV enzyme was purified after the production and some of kinetic properties were investigated for enzyme characterization. Results indicated that the optimum pH and temperature were 8.0 and 40°C with Gly-Pro-pNa as the substrate, respectively. Purified enzyme showed a strong preference for Gly-Pro-pNA as substrate. The enzyme was inhibited by Hg+2, Zn+2, Ca+2, Fe+2 and Mg+2, respectively. It is important and serin protease inhibitor PMSF. The enzyme was strongly inhibited by Diprotin A. Inhibition of the enzyme by jelatin and phenylmethylsulfonyl fluoride (PMSF) were found as similar, and no inhibition was detected with ethylenediaminetatraacetic acid (EDTA).

Keywords
Dipeptidyl Peptidase IV, enzyme inhibition, enzyme kinetic, Lactococcus lactis spp. lactis


Advanced Search


Announcements















    [JAFAG] Yeni Sayımız Basılmıştır JAFAG, 35 (2)

    [JAFAG] New Issue Published JAFAG, 35 (2)



    Call for papers

    To Researcher:

    I am delighted to invite you to disseminate your research findings in Journal of Agricultural Faculty of Gaziosmanpasa University (JAFAG).  I wish to publish original research articles in agricultural sciences. In our journal: rapid manuscript publication and peer review process with online manuscript submission system, quick access of your research results to global audience  with open access system.

    I hope you will contribute your article for publication in JAFAG and thanking you in advance.


    AIMS and SCOPE

    Journal of Agricultural Faculty is scientific, peer reviewed journal and belonged to the Gaziosmanpasa University Faculty of Agriculture since 1985. Journal of Agricultural Faculty of Gaziosmanpasa University aims to publish the national and international scientific paper on agriculture sciences (agricultural economics, animal science, biosystems engineering, field crops, fisheries engineering, horticulture, plant protection, soil science and plant nutrition). The journal is published the third issues in a year from 2014.


    Journal of Agricultural Faculty of Gaziosmanpasa University (JAFAG)

    Journal of Agricultural Faculty of Gaziosmanpasa University (JAFAG) is an international peer-reviewed journal and belonged to the Gaziosmanpasa University Faculty of Agriculture since 1985.

    The journal is open access.

    The journal has both online and printed publishing.

    The journal has a manuscript submisson system.

    There are no page charges.


    INDEXING

    ACADEMIC JOURNALS DATABASE

    AGRICOLA

    ARASTIRMAX

    CAB ABSTRACTS

    CROSSREF

    DIRECTORY OF SCIENCE

    DOAJ (DIRECTORY OF OPEN ACCESS JOURNALS)

    DRJI (DIRECTORY OF RESEARCH JOURNAL INDEXING)

    EBSCO

    EFITA (INTERNATIONAL DIRECTORY OF AGRICULTURE, FOOD AND THE ENVIRONMENT)

    ELECTRONIC JOURNALS LIBRARY

    FAO- AGRIS

    GENAMICS JOURNALSEEK

    GLOBAL IMPACT AND QUALITY FACTOR

    GOOGLE SCHOLAR

    INDEX COPERNICUS

    JOURNAL INDEX

    JOUR INFORMATICS

    OPEN ACCESS LIBRARY

    OPEN J-GATE

    RESEARCH BIBLE

    ROAD (DIRECTORY OF OPEN ACCESS SCHOLARLY  RESOURCES)

    SIS (SCIENTIFIC INDEXING SERVICE)

    SCIENCE CENTRAL

    THE GROVE ONLINE LIBRARY

    TUBITAK-ULAKBIM

    ULRICHSWEB

    WORLDCAT


    [JAFAG] New Issue Published JAFAG, 35 (2)
    Journal of Agricultural Faculty of Gaziosmanpasa University, JAFAG has just published its latest issue (Vol 35, No 2, 2018, JAFAG) at http://ziraatdergi.gop.edu.tr.

    ICV Impact Factor: 4.95 (2012) - 5.94 (2013) - 70.06 (2015) - 72.50(2016)

    We invite you to review the Contents here and then visit journal web site to review articles.

    Thanks for the continuing interest in our work in journal.



Address :Gaziosmanpaşa Üniversitesi Ziraat Fakültesi Dergisi Yayın Ünitesi, Tokat-TÜRKİYE / Adress:Journal of Agricultural Faculty of Gaziosmanpasa University Publishing Unit, Tokat, TURKEY
Telephone :03562521616-2178 / Telephone: +90 356 252 16 16- 2 Fax :03562521488 / Fax:+90 356 252 14 88
Email :ziraatdergi@gop.edu.tr/ e-mail: ziraatdergi@gop.edu.tr

Web Yazılım & Programlama Han Yazılım Bilişim Hizmetleri