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Summary


Rekombinant Soyasistatinin Saflaştırılması ve Papaine ve Balık Proteazlanna Karşı inhibitor Aktivitesinin Saptanması
Rekombinant (r-) soyasistatinin papaine olan inhibitor aktivitesi karakterize edilerek yumurta beyazı sistatini ile karşılaştırılmıştır. E.coli de sentezlenen r-soyasistatin 4.33 kez saf rekombinant protein biçiminde phenyl-Sepharose ve DEAE kolonları ile saflaştırılın ıştır. Yumurta beyazı sistatin ise cm-papain-sepharose afınite kromatograflsi kullanılarak saflaştırılmıştır. Araştırmada, 2 (ig papainiıı %50 sini inhibe etmek için gereken soyasistatin miktarı 0.245 pg ve yumurta beyazı sistatin miktarı ise 0.310 pg dır. r-Soyasıstatın araştırmada kullanılan balık kasındaki otolitik aktivitenin %90 nu inhibe etmiştir.

Turkish Keywords
Soyasistatin yumurta beyazı sistatin proteolitic aktivite protein saflaştırma

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